Manipulation And Analysis Of Dna Using Standard Molecular...

SIB2003 Molecular Biology Practicals Manipulation and analysis of DNA using standard Molecular Biology Techniques. During the course of the next three practical classes you shall be performing a number of techniques in order to isolate and manipulate DNA from bacteria. The practicals are spread over three sessions, the techniques that you will perform are indicated below: Practical 1 Isolation of plasmid DNA from three cultures of E.coli using a method known as the alkaline lysis method. Practical 2 (Part A) Digestion of the plasmid DNA that you have isolated, with restriction enzymes. Practical 2 (Part B) Transformation of bacterial cells (E. coli) with: a) No DNA b) Plasmid DNA c) digested DNA Practical 3 Analysis of DNA from practicals 1 and 2 using the technique of agarose gel electrophoresis and analysis of transfomed E. coli from practical 2 (part B) Whilst the practical classes deal with different techniques, it is important that you write it up as a single laboratory report. The write-up should contain a general introduction written as an overview. The methods and results should be written up as separate sections within the single write-up and the conclusion should be a general summing up exercise where you can discuss the relative success of each practical. Please remember that the write-up should be written up in the passive and in past tense. Further guidelines for the write-up are provided separately and uploaded on Unilearn. Practical 1Show MoreRelatedCloning the Metagenome to Access the Biodiversity of Unculturable Bacteria 1461 Words   |  6 Pagesmicroorganisms. Those bacteria which cannot be grown easily on artificial nutrient media are referred as unculturables. The evidence for the presence of yet to be cultivated bacteria came from the molecular data. The capability to obtain DNA sequence information from an environmental sample by PCR manipulations and direct sequencing allowed identification of these phylogenetically important groups. 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